{"product_id":"pico-custom-assay-development","title":"PICO Custom Assay Development Service","description":"\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003eActome offers a \u003c\/span\u003eprofessional service to design and perform your personalized PICO assays.\u003cspan style=\"font-weight: 400;\"\u003e With PICO you can detect and quantify \u003c\/span\u003e\u003cb\u003eindividual proteins, protein interactions, and post-translational modifications\u003c\/b\u003e\u003cspan style=\"font-weight: 400;\"\u003e with ultra-high sensitivity\u003c\/span\u003e in any liquid biological sample\u003cspan style=\"font-weight: 400;\"\u003e, such as cell lysates, cell extracts, serum, supernatant, or in vitro cell-free conditions.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003eThe service includes the following major steps:\u003c\/span\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli style=\"font-weight: 400;\"\u003e\n\u003cb\u003eSelection of Suitable Antibodies:\u003c\/b\u003e\u003cspan style=\"font-weight: 400;\"\u003e Our team of experts will work with you to identify the most appropriate antibodies for your PICO assay, taking into consideration the target antigen and the intended application.\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli style=\"font-weight: 400;\"\u003e\n\u003cb\u003eConjugation of PICOglue Labels to Your Antibody:\u003c\/b\u003e\u003cspan style=\"font-weight: 400;\"\u003e The PICOglue Labels are conjugated to the antibody using the PICOglue antibody labeling technology.\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli style=\"font-weight: 400;\"\u003e\n\u003cb\u003eQuality Control of the PICOglue-labeled antibody:\u003c\/b\u003e\u003cspan style=\"font-weight: 400;\"\u003e The PICOglue-labeled antibody is assessed for \u003c\/span\u003elabeling efficiency\u003cspan style=\"font-weight: 400;\"\u003e using Agilent's 2100 Bioanalyzer System to ensure that it meets the highest standards of accuracy and reliability. In addition \u003c\/span\u003ethe antibody concentration\u003cspan style=\"font-weight: 400;\"\u003e (labeled antibody molecules\/µL) is also determined using dPCR. \u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli style=\"font-weight: 400;\"\u003e\n\u003cb\u003ePICO Assay and dPCR Run:\u003c\/b\u003e\u003cspan style=\"font-weight: 400;\"\u003e The biological sample is lysed and mixed with the PICOglue-labeled antibodies. After the incubation period the dPCR readout is performed using QIAGEN's QIAcuity dPCR System.\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli style=\"font-weight: 400;\"\u003e\n\u003cb\u003eData Analysis:\u003c\/b\u003e\u003cspan style=\"font-weight: 400;\"\u003e After the dPCR run AMULATOR, our web-based analysis software, performs all the mathematical and statistical evaluation to calculate precise molar concentrations of your target molecule.\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli style=\"font-weight: 400;\"\u003e\n\u003cb\u003eReporting:\u003c\/b\u003e\u003cspan style=\"font-weight: 400;\"\u003e Our experts provide a detailed report of the results of your PICO assay including the \u003c\/span\u003eabsolute concentration of your target molecule (in molar concentration)\u003cspan style=\"font-weight: 400;\"\u003e, graphical representation of the data (box plots), and a step-by-step description of the entire experimental workflow.\u003c\/span\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003e\u003cstrong\u003eContact us\u003c\/strong\u003e below for a quote!\u003c\/span\u003e\u003c\/p\u003e","brand":"PICO BioScience","offers":[{"title":"Default Title","offer_id":44422336577804,"sku":"RDS-000040","price":25000.0,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0042\/1026\/2051\/files\/Veritcalarrowwithbox.png?v=1683553244","url":"https:\/\/pico-bioscience.myshopify.com\/products\/pico-custom-assay-development","provider":"PICO BioScience GmbH","version":"1.0","type":"link"}